Composite

Part:BBa_K2959007:Design

Designed by: Alejandro Aguirre Hern�ndez   Group: iGEM19_Tec-Chihuahua   (2019-10-07)


Expressible Wheat antimicrobial peptide 1b


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 261


Design Notes

The sequence of WAMP1b is preceded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.


Source

The composite part was designed using the following parts previously added to the Registry: BBa_R0010, BBa_B0034, and BBa_B0015. The amino acid sequence for WAMP1b was retrieved from Odintsova et al., 2009.

References

1. Odintsova, T. I., Vassilevski, A. A., Slavokhotova, A. A., Musolyamov, A. K., Finkina, E. I., Khadeeva, N. V., … Egorov, T. A. (2009). A novel antifungal hevein-type peptide fromTriticum kiharaeseeds with a unique 10-cysteine motif. FEBS Journal, 276(15), 4266–4275. doi: 10.1111/j.1742-4658.2009.07135.x